کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8038022 | 1518319 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Compressed sensing electron tomography of needle-shaped biological specimens - Potential for improved reconstruction fidelity with reduced dose
ترجمه فارسی عنوان
توموگرافی الکترونی سنجش فشرده نمونه های بیولوژیکی سوزنی - توان بالقوه برای بهبود وفاداری بازسازی با کاهش دوز
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
توموگرافی الکترونی، وضوح ایزوتروپیک، سنجش فشرده، علم زندگی،
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
فناوری نانو (نانو تکنولوژی)
چکیده انگلیسی
Electron tomography is an invaluable method for 3D cellular imaging. The technique is, however, limited by the specimen geometry, with a loss of resolution due to a restricted tilt range, an increase in specimen thickness with tilt, and a resultant need for subjective and time-consuming manual segmentation. Here we show that 3D reconstructions of needle-shaped biological samples exhibit isotropic resolution, facilitating improved automated segmentation and feature detection. By using scanning transmission electron tomography, with small probe convergence angles, high spatial resolution is maintained over large depths of field and across the tilt range. Moreover, the application of compressed sensing methods to the needle data demonstrates how high fidelity reconstructions may be achieved with far fewer images (and thus greatly reduced dose) than needed by conventional methods. These findings open the door to high fidelity electron tomography over critically relevant length-scales, filling an important gap between existing 3D cellular imaging techniques.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ultramicroscopy - Volume 160, January 2016, Pages 230-238
Journal: Ultramicroscopy - Volume 160, January 2016, Pages 230-238
نویسندگان
Zineb Saghi, Giorgio Divitini, Benjamin Winter, Rowan Leary, Erdmann Spiecker, Caterina Ducati, Paul A. Midgley,