کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8038105 1518324 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nanoscale imaging of the growth and division of bacterial cells on planar substrates with the atomic force microscope
ترجمه فارسی عنوان
تصویربرداری از نانوساختار رشد و تقسیم سلول های باکتری بر روی زیربناهای ورقه ای با میکروسکوپ نیروی اتمی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد فناوری نانو (نانو تکنولوژی)
چکیده انگلیسی
With the use of the atomic force microscope (AFM), the Nanomicrobiology field has advanced drastically. Due to the complexity of imaging living bacterial processes in their natural growing environments, improvements have come to a standstill. Here we show the in situ nanoscale imaging of the growth and division of single bacterial cells on planar substrates with the atomic force microscope. To achieve this, we minimized the lateral shear forces responsible for the detachment of weakly adsorbed bacteria on planar substrates with the use of the so called dynamic jumping mode with very soft cantilever probes. With this approach, gentle imaging conditions can be maintained for long periods of time, enabling the continuous imaging of the bacterial cell growth and division, even on planar substrates. Present results offer the possibility to observe living processes of untrapped bacteria weakly attached to planar substrates.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ultramicroscopy - Volume 154, July 2015, Pages 29-36
نویسندگان
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