کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8038329 1518335 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Correlated cryo-fluorescence and cryo-electron microscopy with high spatial precision and improved sensitivity
ترجمه فارسی عنوان
میکروسکوپ کریو فلورسانس و میکروسکوپ الکترونی کریو با دقت مکانی بالا و حساسیت بهبود یافته
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد فناوری نانو (نانو تکنولوژی)
چکیده انگلیسی
Performing fluorescence microscopy and electron microscopy on the same sample allows fluorescent signals to be used to identify and locate features of interest for subsequent imaging by electron microscopy. To carry out such correlative microscopy on vitrified samples appropriate for structural cryo-electron microscopy it is necessary to perform fluorescence microscopy at liquid-nitrogen temperatures. Here we describe an adaptation of a cryo-light microscopy stage to permit use of high-numerical aperture objectives. This allows high-sensitivity and high-resolution fluorescence microscopy of vitrified samples. We describe and apply a correlative cryo-fluorescence and cryo-electron microscopy workflow together with a fiducial bead-based image correlation procedure. This procedure allows us to locate fluorescent bacteriophages in cryo-electron microscopy images with an accuracy on the order of 50 nm, based on their fluorescent signal. It will allow the user to precisely and unambiguously identify and locate objects and events for subsequent high-resolution structural study, based on fluorescent signals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ultramicroscopy - Volume 143, August 2014, Pages 24-32
نویسندگان
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