کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8066 575 2010 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Polyion complex stability and gene silencing efficiency with a siRNA-grafted polymer delivery system
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Polyion complex stability and gene silencing efficiency with a siRNA-grafted polymer delivery system
چکیده انگلیسی

An siRNA-grafted polymer through disulfide linkage was prepared to improve the physicochemical properties and transfection efficacies of the polyion complexes (PICs) as a nanocarrier of siRNA. The siRNA-grafted polymer formed stable PICs due to its larger numbers and higher density of anionic charges compared with monomeric siRNA, leading to effective internalization by cultured cells. Following the endosomal escape of the PIC, the disulfide linkage of the siRNA-grafted polymer allowed efficient siRNA release from the PIC under intracellular reductive conditions. Consequently, the PIC from the siRNA-grafted polymer showed a potent gene silencing effect without cytotoxicity or immunogenicity, demonstrating a promising feature of the siRNA-grafted polymer to construct the PIC-based nanocarrier for in vivo siRNA delivery.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 31, Issue 31, November 2010, Pages 8097–8105
نویسندگان
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