کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
807811 1468293 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Real-time electrical detection of the formation and destruction of lipid bilayers on silicon nanowire devices
ترجمه فارسی عنوان
تشخیص الکتریکی در زمان واقعی تشکیل و تخریب دو لایه لیپید بر روی دستگاه های نانوسیم سیلیکون
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی

Silicon nanowire (Si NW) two-terminal devices were fabricated to electrically probe the real-time formation and destruction of lipid bilayers. A liposome solution, containing the same ratio of zwitterionic/anionic lipids that are present in an Escherichia coli cell membrane, was applied to the NW devices. Lipid bilayer formation on the Si NWs was detected in-situ by observing electrical resistance changes complemented by confocal fluorescence microscopy imaging. The formation of lipid bilayers resulted in a 1% to 2% decrease in device current, consistent with the negative gating effect of the lipids on the NW surface. The devices demonstrated a ≈ 1 min electrical response time to lipid encapsulation. Removal of the lipid layer was achieved by exposing the devices to a detergent, which resulted in NW conductance returning to its original value with a ≈ 2 min recovery time. The lipid bilayer coated Si NWs demonstrate a novel platform to enable in-situ electrical probing of bacterial cell membrane mechanisms, interactions, and reactions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensing and Bio-Sensing Research - Volume 4, June 2015, Pages 103–108
نویسندگان
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