کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8086425 1521813 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cell diameter doesn't affect lipid productivity of Chlorococcum littorale
ترجمه فارسی عنوان
قطر سلول تاثیری بر میزان تولید چربی های کلوروکوکومتری ندارد
موضوعات مرتبط
مهندسی و علوم پایه مهندسی انرژی انرژی های تجدید پذیر، توسعه پایدار و محیط زیست
چکیده انگلیسی
We hypothesized that cells with different diameter have different division rates, which could affect lipid productivity (lipid content × biomass productivity). In the present work we assessed the influence of cell diameter, as a sorting parameter, on both biomass and lipid productivity of Chlorococcum littorale. Prior to sorting, cells were grown in a batch-wise nitrogen run-out including a long nitrogen depleted phase (N −) to stop cell division, thus only having vegetative cells (Pre-sorting). Cell sorting was done at the end of this N − phase using FACS (fluorescence assisted cell sorting) based on forward scatter as a proxy for diameter (size ranges (μm): 5-6 (small), 8-9 (medium), 11-14 (large) and 5-14 (control)). The sorting was done in 2 pools: multiple-cell (100 cells) and single-cell. After sorting, cells were recovered under low-light for 2 weeks, and used to start the Post-sorting experiment (analogous to Pre-sorting). The populations derived from different sorted pools, single-cell and multiple-cell, showed similar size distributions after re-growth. No difference was observed in biomass and lipid productivities among Post-sorting cells and when compared to Pre-sorting cells under nitrogen depletion. We concluded that cellular size had no effect on both biomass and lipid productivity of C. littorale.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Algal Research - Volume 19, November 2016, Pages 333-341
نویسندگان
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