کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8086425 | 1521813 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cell diameter doesn't affect lipid productivity of Chlorococcum littorale
ترجمه فارسی عنوان
قطر سلول تاثیری بر میزان تولید چربی های کلوروکوکومتری ندارد
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی انرژی
انرژی های تجدید پذیر، توسعه پایدار و محیط زیست
چکیده انگلیسی
We hypothesized that cells with different diameter have different division rates, which could affect lipid productivity (lipid content Ã biomass productivity). In the present work we assessed the influence of cell diameter, as a sorting parameter, on both biomass and lipid productivity of Chlorococcum littorale. Prior to sorting, cells were grown in a batch-wise nitrogen run-out including a long nitrogen depleted phase (N â) to stop cell division, thus only having vegetative cells (Pre-sorting). Cell sorting was done at the end of this N â phase using FACS (fluorescence assisted cell sorting) based on forward scatter as a proxy for diameter (size ranges (μm): 5-6 (small), 8-9 (medium), 11-14 (large) and 5-14 (control)). The sorting was done in 2 pools: multiple-cell (100 cells) and single-cell. After sorting, cells were recovered under low-light for 2 weeks, and used to start the Post-sorting experiment (analogous to Pre-sorting). The populations derived from different sorted pools, single-cell and multiple-cell, showed similar size distributions after re-growth. No difference was observed in biomass and lipid productivities among Post-sorting cells and when compared to Pre-sorting cells under nitrogen depletion. We concluded that cellular size had no effect on both biomass and lipid productivity of C. littorale.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Algal Research - Volume 19, November 2016, Pages 333-341
Journal: Algal Research - Volume 19, November 2016, Pages 333-341
نویسندگان
Iago Teles Dominguez Cabanelas, Carolina Fernandes, Dorinde M.M. Kleinegris, René H. Wijffels, Maria J. Barbosa,