کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8087019 | 1521816 | 2016 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An improved ARG7-based selection cassette with highly efficient transformation rates and a small size suitable for complex expression constructs
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی انرژی
انرژی های تجدید پذیر، توسعه پایدار و محیط زیست
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: An improved ARG7-based selection cassette with highly efficient transformation rates and a small size suitable for complex expression constructs An improved ARG7-based selection cassette with highly efficient transformation rates and a small size suitable for complex expression constructs](/preview/png/8087019.png)
چکیده انگلیسی
In Chlamydomonas reinhardtii, the ARG7 locus constitutes one of the first and most widely used selection markers. Until now it has been available only as a large, intractable genomic fragment unsuitable for incorporation in complex expression constructs and exhibiting poor transformation efficiency. Here we succeed in significantly improving the ARG7 nuclear marker by placing the short arginosuccinate lyase (ASL) cDNA under its own promoter and 5â² UTR and by including the first ASL intron. We test the new ARG7 marker in three different arginine auxotrophic strains and show highly superior transformation efficiencies compared to the conventional genomic ARG7 marker. The plasmid carrying the shorter ARG7 selection cassette has been deposited at the Chlamydomonas Resource Center under the name pHR11.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Algal Research - Volume 16, June 2016, Pages 383-386
Journal: Algal Research - Volume 16, June 2016, Pages 383-386
نویسندگان
Hussam Hassan Nour-Eldin, Elizabeth A. Specht, Stephen P. Mayfield,