کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8208513 | 1532057 | 2018 | 19 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development and optimization of a novel automated loop method for production of [11C]nicotine
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موضوعات مرتبط
مهندسی و علوم پایه
فیزیک و نجوم
تشعشع
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A novel, rapid, and automated loop method for the synthesis of [11C]nicotine was developed and optimized. The method involves, a reaction of the precursor, (+) nornicotine or (-) nornicotine, with a gas-phase produced [11C]CH3I in an 800â¯ÂµL loop at 75â¯Â°C for 5â¯min followed by a semi-preparatory Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) purification. The optimized synthesis and purification process was complete in <â¯30â¯min and produced [11C]nicotine with >â¯99.9% Radiochemical Purity (RCP), no [11C]CH3I, no (+) nornicotine, 105â¯mCi/µmole specific activity, 7.0 - 7.2â¯pH, and 16.6% ethanol. The current method can be optimized, to reduce the ethanol content (<10%), and can be translated to a cGMP production of [11C]nicotine for human clinical trials.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Applied Radiation and Isotopes - Volume 140, October 2018, Pages 76-82
Journal: Applied Radiation and Isotopes - Volume 140, October 2018, Pages 76-82
نویسندگان
Arijit Ghosh, Karen Woolum, Michael V. Knopp, Krishan Kumar,