کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8209822 | 1532100 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparison of results of the manual and automated scoring of micronucleus frequencies in 60Co-irradiated peripheral blood lymphocytes for triage dosimetry
ترجمه فارسی عنوان
مقایسه نتایج حاصل از دستیابی و ارزیابی اتوماتیک فرکانس های میکرونوکلئوس در لنفوسیت های خون محیطی 60 کروموزومی برای دوزیمتری تریاژ
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کلمات کلیدی
بیوداسیمتری، اتوماسیون، خون، میکرونوکلئیک،
موضوعات مرتبط
مهندسی و علوم پایه
فیزیک و نجوم
تشعشع
چکیده انگلیسی
Scoring micronuclei in the peripheral blood lymphocytes of individuals exposed to ionizing radiation is a rapid biodosimetry assay. Peripheral blood lymphocytes from five individuals were exposed in vitro to 0-5 Gy of 60Co γ-radiation at a dose rate of 0.76 Gy/min. The blood cultures were initiated with RPMI-1640 (80%) supplemented with FBS (20%), stimulated with mitogen and incubated at 37 °C for 44 h. At the 44th hour, cytochalasin-B (6 µg/mL) was added, and the cultures were incubated for 28 h more. The cells were harvested with a pre-chilled hypotonic solution (0.075 M) and fixed with a Carnoy's solution (methanol/acetic acid 5:1). Giemsa- and propidium-iodide-stained cells affixed to slides for microscopy were scored manually and automatically with the micronucleus scoring software from MetaSystems. The micronucleus frequencies determined in the Giemsa-stained cells by manual and automated scoring were 23.6% different (P<0.0001) with an efficiency of 24.9%. Slides stained with propidium iodide are a better choice for automated scoring than Giemsa-stained ones.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Applied Radiation and Isotopes - Volume 97, March 2015, Pages 70-77
Journal: Applied Radiation and Isotopes - Volume 97, March 2015, Pages 70-77
نویسندگان
G. Tamizh Selvan, N.K. Chaudhury, P. Venkatachalam,