کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8248020 1533318 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Use of micro-computed tomography to evaluate the effects of exercise on preventing the degeneration of articular cartilage in tail-suspended rats
ترجمه فارسی عنوان
استفاده از توموگرافی میکرو محاسباتی برای ارزیابی اثرات ورزش بر جلوگیری از انحطاط غضروف مفصلی در موشهای صحرایی باقیمانده
کلمات کلیدی
غضروف مفصلی، ورزش، هیستومورفولوژی، توموگرافی کامپیوتری میکروسکوپی، تخلیه،
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات علوم فضا و نجوم
چکیده انگلیسی
Space flight has been shown to induce bone loss and muscle atrophy, which could initiate the degeneration of articular cartilage. Countermeasures to prevent bone loss and muscle atrophy have been explored, but few spaceflight or ground-based studies have focused on the effects on cartilage degeneration. In this study, we investigated the effects of exercise on articular cartilage deterioration in tail-suspended rats. Thirty-two female Sprague-Dawley rats were randomly divided into four groups (n=8 in each): tail suspension (TS), tail suspension plus passive motion (TSP), tail suspension plus active exercise (TSA), and control (CON) groups. In the TS, TSP, and TSA groups, the rat hindlimbs were unloaded for 21 days by tail suspension. Next, the cartilage thickness and volume, and the attenuation coefficient of the distal femur were evaluated by micro-computed tomography (μCT). Histological analysis was used to assess the surface integrity of the cartilage, cartilage thickness, and chondrocytes. The results showed that: (1) the cartilage thickness on the distal femur was significantly lower in the TS and TSP groups compared with the CON and TSA groups; (2) the cartilage volume in the TS group was significantly lower compared with the CON, TSA, and TSP groups; and (3) histomorphology showed that the chondrocytes formed clusters where the degree of matrix staining was lower in the TS and TSP groups. There were no significant differences between any of these parameters in the CON and TSA groups. The cartilage thickness measurements obtained by μCT and histomorphology correlated well. In general, tail suspension could induce articular cartilage degeneration, but active exercise was effective in preventing this degeneration in tail-suspended rats.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Life Sciences in Space Research - Volume 6, July 2015, Pages 15-20
نویسندگان
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