کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8252507 1533508 2015 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Microbial biofilm study by synchrotron X-ray microscopy
ترجمه فارسی عنوان
مطالعه بیوفیلم میکروبی توسط میکروسکوپ اشعه ایکس میکروسکوپی
موضوعات مرتبط
مهندسی و علوم پایه فیزیک و نجوم تشعشع
چکیده انگلیسی
Microbial biofilm has already being used to remove metals and other pollutants from wastewater. In this sense, our proposal was to isolate and cultivate bacteria consortia from mangrove's sediment resistant to Zn (II) and Cu (II) at 50 mg L−1 and to observe, through synchrotron X-ray fluorescence microscopy (microXRF), whether the biofilm sequestered the metal. The biofilm area analyzed was 1 mm2 and a 2D map was generated (pixel size 20×20 μm2, counting time 5 s/point). The biofilm formation and retention followed the sequence Zn>Cu. Bacterial consortium zinc resistant formed dense biofilm and retained 63.83% of zinc, while the bacterial consortium copper resistant retained 3.21% of copper, with lower biofilm formation. Dehydrogenase activity of Zn resistant bacterial consortium was not negatively affect by 50 mg ml−1 zinc input, whereas copper resistant bacterial consortium showed a significant decrease on dehydrogenase activity (50 mg mL−1 of Cu input). In conclusion, biofilm may protect bacterial cells, acting as barrier against metal toxicity. The bacterial consortia Zn resistant, composed by Nitratireductor spp. and Pseudomonas spp formed dense biofilm and sequestered metal from water, decreasing the metal bioavailability. These bacterial consortia can be used in bioreactors and in bioremediation programs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Radiation Physics and Chemistry - Volume 116, November 2015, Pages 116-119
نویسندگان
, , , , ,