Method for the simultaneous determination of free/protein malondialdehyde and lipid/protein hydroperoxides

► Lipid peroxidation assessment is difficult because of its often unstable products. ► An array of inefficient methods exists for the measurement of these products. ► These methods make it almost impossible to judge the various products. ► To overcome these methodological difficulties we developed a simple protocol. ►Our method simultaneously quantifies free MDA, protein-MDA, LOOH, and protein-OOH.