Robust protein nitration contributes to acetaminophen-induced mitochondrial dysfunction and acute liver injury

► Many mitochondrial proteins were nitrated after acetaminophen (APAP) exposure. ► Nitrated proteins were purified and their identities determined by mass spectrometry. ► Nitrated mitochondrial proteins exhibited decreased activity. ► N-acetylcysteine prevented APAP-induced mitochondrial protein nitration. ► Protein nitration correlated with decreased activity and hepatotoxicity.