Quantitative site-specific reactivity profiling of S-nitrosylation in mouse skeletal muscle using cysteinyl peptide enrichment coupled with mass spectrometry

► A quantitative proteomic approach for site-specific profiling of S-nitrosylation was optimized. ► Protein- and peptide-level enrichment protocols provided comparable specificity and coverage. ► Quantitative reactivity profiling identified 488 S-nitrosylation sites from mouse muscle. ► Sites sensitive to S-nitrosylation and pathways susceptible to modification were revealed.