Membrane association of peroxiredoxin-2 in red cells is mediated by the N-terminal cytoplasmic domain of band 3

► The cytoplasmic domain of B3 is the docking site for PRDX2. ► Binding of PRDX2 to the cytoplasmic domain of B3 determines a conformational change. ► The complex PRDX2:cytoplasmic domain of B3 exhibits peroxidase activity. ► The first 11 amino acids of B3 cytoplasmic domain are important for PRDX2 binding.