Determination of iohexol in human serum by a semi-automated liquid chromatography tandem mass spectrometry method

This method accurately and precisely quantifies iohexol in 50 μL of serum, enabling determination of mGFR by iohexol clearance. The method is highly correlated to a reference method. Use of an automated liquid handler reduces labor-intensive, manual sample preparation steps. The stability of this analyte and the robustness of this assay fit well within our clinical workflow and we have successfully applied this method to determine mGFR in pediatric patients.