Development of an enzymatic assay for sphingomyelin with rapid and automatable performances: Analysis in healthy subjects and coronary heart disease patients

► We have described a rapid and automatable enzymatic assay for sphingomyelin (SM). ► Our results suggest that this assay could be applied to clinical laboratory testing. ► Our SM measurement results are consistent with those previously reported. ► The serum SM level is higher in patients with acute coronary syndrome. ► The serum SM level is correlated with the serum magnesium concentration.