کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8318044 | 1538963 | 2018 | 45 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
RANKL, Ephrin-Eph and Wnt10b are key intercellular communication molecules regulating bone remodeling in autologous transplanted goldfish scales
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کلمات کلیدی
Wnt10bnuclear factor of activated T cells c1CTSKNFATc1osterixCOL1A1MMP9ISHPFATRAPRANKLIn situ hybridization - Hybridization در محلCell-cell communication - ارتباط سلول-سلولOsteoblasts - استئوبلاست هاOsteoclasts - استخوانکاه tartrate-resistant acid phosphatase - اسید فسفاتاز مقاوم در برابر تارتاتOsx - اکسIHC - ایمونوهیستوشیمیImmunohistochemistry - ایمونوهیستوشیمیbone remodeling - بازسازی استخوانRank - رتبهLuria-Bertani - لواریا بارتانیMatrix metalloproteinase 9 - ماتریکس متالوپروتئیناز 9paraformaldehyde - پارافرمالدهیدCathepsin K - کتهفسین کType I collagen - کلاژن نوع IReceptor activator of nuclear factor κB - گیرنده گیرنده فاکتور هسته ای κBreceptor activator of NF-κB ligand - گیرنده گیرنده لیگاند NF-κB
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: RANKL, Ephrin-Eph and Wnt10b are key intercellular communication molecules regulating bone remodeling in autologous transplanted goldfish scales RANKL, Ephrin-Eph and Wnt10b are key intercellular communication molecules regulating bone remodeling in autologous transplanted goldfish scales](/preview/png/8318044.png)
چکیده انگلیسی
This study aimed to investigate the precise data of gene expression, functions, and chronological relationships amongst communication molecules involved in the bone remodeling process with an in vivo model using autologous transplanted scales of goldfish. Autotransplantation of methanol-fixed cell-free scales triggers scale resorption and regeneration, as well as helps elucidate the process of bone remodeling. We investigated osteoclastic markers, osteoblastic markers, and gene expressions of communicating molecules (RANKL, ephrinB2, EphB4, EphA4, Wnt10b) by qPCR, in situ hybridization for Wnt10b, and immunohistochemistry for EphrinB2 and EphA4 proteins to elucidate the bone remodeling process. Furthermore, functional inhibition experiments for the signaling of ephrinB2/Eph, ephrin/EphA4, and Wnt10b using specific antibodies, revealed that these proteins are involved in key signaling pathways promoting normal bone remodeling. Our data suggests that the remodeling process comprises of two successive phases. In the first absorption phase, differentiation of osteoclast progenitors by RANKL is followed by the bone absorption by mature, active osteoclasts, with the simultaneous induction of osteoblast progenitors by multinucleated osteoclast-derived Wnt10b, and proliferation of osteoblast precursors by ehprinB2/EphB4 signaling. Subsequently, during the second formation phase, termination of bone resorption by synergistic cooperation occurs, with downregulation of RANKL expression in activated osteoblasts and Ephrin/EphA4-mediated mutual inhibition between neighboring multinucleated osteoclasts, along with simultaneous activation of osteoblasts via forward and reverse EphrinB2/EphB4 signaling between neighboring osteoblasts. In addition, the present study shows that autologous transplantation of methanol-fixed cell-free scale is an ideal in vivo model to study bone remodeling.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 225, November 2018, Pages 46-58
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 225, November 2018, Pages 46-58
نویسندگان
Yuya Tazaki, Kayo Sugitani, Kazuhiro Ogai, Isao Kobayashi, Haruki Kawasaki, Takafumi Aoyama, Nobuo Suzuki, Yoshiaki Tabuchi, Atsuhiko Hattori, Kei-ichiro Kitamura,