کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8319094 | 1539276 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Glyphosate-based herbicide exposure causes antioxidant defence responses in the fruit fly Drosophila melanogaster
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Glyphosate-based herbicide exposure causes antioxidant defence responses in the fruit fly Drosophila melanogaster Glyphosate-based herbicide exposure causes antioxidant defence responses in the fruit fly Drosophila melanogaster](/preview/png/8319094.png)
چکیده انگلیسی
Glyphosate is a non-selective and post-emergent herbicide that affects plant growth. Animal exposure to this herbicide can lead to adverse effects, such as endocrine disruption, oxidative stress and behavioural disorders. Drosophilids have been utilized previously as an effective tool in toxicological tests. In the present study, the effects of a glyphosate-based herbicide (Roundup [Original]) were investigated regarding oxidative stress, the antioxidant defence system and acetylcholinesterase (AChE) activity in Drosophila melanogaster. Flies (of both genders) that were 1 to 3Â days old were exposed to different glyphosate concentrations (0.0Â mg/LÂ =Â control, 1.0Â mg/L, 2.0Â mg/L, 5.0Â mg/L and 10.0Â mg/L) in the diet for 24Â h and 96Â h. After the exposure periods, reactive oxygen species (ROS) levels, antioxidant capacity against peroxyl radicals (ACAP) and lipid peroxidation (LPO) levels were quantified. In addition, the mRNA expression of antioxidant genes (i.e., keap1, sod, sod2, cat, irc, gclc, gclm, gss, trxt, trxr-1 and trxr-2) was evaluated via RT-PCR. Additionally, AChE activity was evaluated only after the 96Â h exposure period. The results indicated that Roundup exposure leads to a reduction in ROS levels in flies exposed for 96Â h. ACAP levels and gene expression of the antioxidant defence system exhibited an increase from 24Â h, while LPO did not show any significant alterations in both exposure periods. AChE activity was not affected following Roundup exposure. Our data suggest that Roundup exposure causes an early activation of the antioxidant defence system in D. melanogaster, and this can prevent subsequent damage caused by ROS.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volumes 185â186, JulyâAugust 2016, Pages 94-101
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volumes 185â186, JulyâAugust 2016, Pages 94-101
نویسندگان
Lais Mattos de Aguiar, Fernanda Hernandes Figueira, Marco Silva Gottschalk, Carlos Eduardo da Rosa,