کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8319125 | 1539289 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Variability in antioxidant/detoxification enzymes of Labeo rohita exposed to an azo dye, acid black (AB)
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The aim of the present study was to evaluate effect of a highly toxic azo dye, acid black (AB) (CI: 20470, 96Â h LC50Â =Â 10Â mg/L) on the biochemical responses of Labeo rohita. Antioxidant/detoxification enzymes such as glutathione-S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD), acetylcholinesterase (AChE), lactate dehydrogenase (LDH), succinate dehydrogenase (SDH), acid phosphatase (AcP), alkaline phosphatase (AKP), alanine transaminase (ALT) and aspartate transaminase (AST) were determined in liver, kidney, gill, muscle and brain of L. rohita after 96Â h exposure to 6Â mg/L (LC10), 8Â mg/L (LC30) and 10Â mg/L (LC50) of dye with an aim to find out the target tissue and biomarker enzyme for AB. The fish were then kept for a recovery period of 90Â days, and activity of the selected enzymes was determined at the end of this period. Present dye altered the activities of all these enzymes in the selected tissues of the experimental fish in a dose-dependent manner. SOD was the maximally affected enzyme, and liver was the most affected tissue. The results indicate that AB is very toxic to L. rohita as there was a significant effect of even 6Â mg/L dose of the dye and the toxicity prolonged for a long time because the fish was not able to recover from the stress even 90Â days after the exposure. The study suggests that SOD can be used as a biomarker enzyme and liver is the target tissue for AB.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 167, January 2015, Pages 108-116
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 167, January 2015, Pages 108-116
نویسندگان
Satinder Kaur, Arvinder Kaur,