کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8326751 | 1540196 | 2018 | 49 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Improvement of enzymological properties of pepsin by chemical modification with chitooligosaccharides
ترجمه فارسی عنوان
بهبود خواص آنزیمولوژیک پپسین با اصلاح شیمیایی با کیتولیگوساکاریدها
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
In order to improve the enzymological properties and to enhance the stability of pepsin (PP, EC 3.4.23.1), chitooligosaccharide (COS) was used to chemically modify enzyme molecules of PP. The results showed that one protein band appeared on the PAGE diagram, indicating that the enzyme samples reached electrophoresis purity after purification. The molecular weights of the enzymes before (PP) and after (COS-PP) modification were 38.68â¯KD and 49.55â¯KD, respectively. The Km and Vmax of PP were 2.40â¯mg/mL and 1.1â¯Ãâ¯106â¯mg/(mgâ¯Proâ¯min). The Km and Vmax of COS-PP were 4.44â¯mg/mL and 8.3â¯Ãâ¯105â¯mg/(mgâ¯Proâ¯min), respectively. The optimal pH values of PP and COS-PP were 1.5-2.0. The optimal temperatures of PP and COS-PP were 57â¯Â°C and 59â¯Â°C, respectively. After being incubated at 60â¯Â°C for 1â¯h, the residual activities of PP and COS-PP solutions were 26.56% and 74.72%, respectively. After being incubated at 65â¯Â°C for 1â¯h, the residual activities of PP and COS-PP solutions were 14.74% and 46.40%, respectively. After being kept at room temperature (25â¯Â°C) or 4â¯Â°C refrigerator for 4â¯days, PP solution had basically no activity, whereas the residual activities of COS-PP solutions were 5.40% and 33.94%, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 118, Part A, 15 October 2018, Pages 216-227
Journal: International Journal of Biological Macromolecules - Volume 118, Part A, 15 October 2018, Pages 216-227
نویسندگان
Chunyi Li, Zhuolie Huang, Li Dong, Xiaolin Liu,