کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8326786 | 1540196 | 2018 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of a novel polysaccharide from Ganoderma lucidum and its absorption mechanism in Caco-2 cells and mice model
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Fungal polysaccharides are mainly taken orally, so it is very important to study the absorption mechanism of which in vivo for understanding its biological activities. In this work, a polysaccharide fraction (GLP) was purified from G. lucidum by water extraction and alcohol precipitation. Physicochemical characterization indicated that GLP had a molecular weight of 108â¯kDa and consisted of glucose (35.56%), xylose (40.11%), rhamnose (16.45%) and l-arabinose (7.88%) in a molar radio of 4.51: 5.09: 2.88: 1.0. GLP was taken by Caco-2 cells through the pathway of macropinocytosis and it was a well-absorbed biological macromolecule testified by Papp value. Sugar transporters (SGLT1 and GLUT2) played different roles at different absorption stages of GLP, and the absorption of GLP during digestion in mice model was a time-dependent process. All these results could contribute to explore the absorption mechanism of polysaccharides in body.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 118, Part A, 15 October 2018, Pages 320-326
Journal: International Journal of Biological Macromolecules - Volume 118, Part A, 15 October 2018, Pages 320-326
نویسندگان
Zichao Wang, Huiru Zhang, Yingbin Shen, Xiaoxiao Zhao, Xueqin Wang, Jiaqi Wang, Kun Fan, Xiaobei Zhan,