کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8327096 1540197 2018 25 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structural characterization and α-glucosidase inhibitory activity of polysaccharides extracted from Chinese traditional medicine Huidouba
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Structural characterization and α-glucosidase inhibitory activity of polysaccharides extracted from Chinese traditional medicine Huidouba
چکیده انگلیسی
In this research, the crude polysaccharides were extracted from Chinese traditional medicine Huidouba and then isolated and purified by DEAE-52 cellulose and Sephadex G-100 chromatographic columns. The hypoglycemic activities of two fractions were determined by α-glucosidase inhibitory activity in vitro. The fraction HDBP-3A showed the best α-glucosidase inhibitory activity with an IC50 of 0.0075 mg/mL, which was lower than the acarbose positive control (IC50 of 0.092 mg/mL). The structural features of HDBP-3A was investigated by the high-performance gel permeation chromatography (HPGPC), gas chromatography(GC), UV spectroscopy, FT-IR, periodate oxidation and Smith degradation. The results demonstrated that the HDBP-3A with a molecular weight of 86,749 Da mainly composed of l-rhamnose, d-fucose, l-arabinose, d-xylose, d-mannose, d-glucose and d-galactose with the molar ratio of 2.33:1.58:1.00:1.37:4.86:4.00:7.30. The branches of HDBP-3A were composed of (1 → 4)-linked, (1 → 6)-linked, (1 → 2)-linked or (1 → 3)-linked. The IR spectrum implied that β-pyranoside linkage and α-configurations existed in HDBP-3A simultaneously.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 117, 1 October 2018, Pages 815-819
نویسندگان
, , , , , ,