کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8327152 1540200 2018 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nanofibrous silk fibroin/reduced graphene oxide scaffolds for tissue engineering and cell culture applications
ترجمه فارسی عنوان
فیبرن ابریشمی نانوفیلین / داربست اکسید گرافین کاهش یافته برای مهندسی بافت و کاربردهای کشت سلولی
کلمات کلیدی
فیبرن ابریشم، کاهش اکسید گرافین، بیومتریک کامپوزیت نانوفیبری،
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
Graphene and silk fibroin (SF) have been extensively investigated in the literature. Hybrid scaffolds of SF and graphene combine the properties of both of the materials and provide promising applications for tissue engineering purposes. In this study, reduced graphene oxide (RGO) (0.5%, 1.0% and 2.0% (w/v)) was incorporated into SF and fabricated into composite nanofibers through electrospinning. The fibers were characterized and analyzed by SEM, XRD, FTIR, TGA, circular dichroism analysis, contact angle measurements and tensile tests. Here, we document that the presence of RGO increases intermolecular forces between RGO and SF molecular chains in the SF matrix, which results in an increased silk II content. Upon the incorporation of RGO, thermal stability and mechanical properties of the fibers significantly improved. Furthermore, in-vitro findings showed that composite nanofibers supported cell viability and were hemocompatible. Finally, bone marrow mesenchymal stem cells were induced osteogenically on electrospun SF/RGO mats for 30 days, which showed that the substrate supported osteogenic differentiation. In this study, a feasible method is proposed to generate biocompatible and versatile SF/RGO-composite nanofibers that can influence biomedical applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 114, 15 July 2018, Pages 77-84
نویسندگان
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