کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8327410 | 1540199 | 2018 | 39 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enhanced biosynthesis of dextransucrase: A multivariate approach to produce a glucosyltransferase for biocatalysis of sucrose into dextran
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Enhanced biosynthesis of dextransucrase: A multivariate approach to produce a glucosyltransferase for biocatalysis of sucrose into dextran Enhanced biosynthesis of dextransucrase: A multivariate approach to produce a glucosyltransferase for biocatalysis of sucrose into dextran](/preview/png/8327410.png)
چکیده انگلیسی
The current study reported the statistically designed experimental method to enhance the biocatalytic efficacy of dextransucrase from Weissella confusa. Various environmental and nutritional parameters were optimized using multiple responses under submerged fermentation environment. Statistical models were constructed to screen the influence of nine factors on the biocatalysis of dextransucrase. Among them, fermentation time, pH, sucrose and peptone exhibited significant probability (Pâ¯<â¯0.05) and are considered as substantial constituents in accordance with Plackett-Burman design. Central composite design was further implemented to optimize the levels of selected variables for maximum enzyme yield. The predicted optimum conditions were pH of 7.5 under fermentation time of 8â¯h with 30.0â¯gâ¯lâ1 sucrose and 1.0â¯gâ¯lâ1 peptone. The overall enzyme yield increased from 11.4â¯DSUâ¯mlâ1 to 52.75â¯DSUâ¯mlâ1 with 4.62-fold upsurge after the implementation of the statistical models. Furthermore, SEM analysis showed the biocatalytic conversion of sucrose into highly porous dextran when utilizing dextransucrase. The biopolymer produced under the current optimized model could be utilized as an emulsifying, gelling, stabilizing and thickening agent in food industry.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 115, August 2018, Pages 776-785
Journal: International Journal of Biological Macromolecules - Volume 115, August 2018, Pages 776-785
نویسندگان
Syeda Bushra Zafar, Tayyaba Asif, Shah Ali Ul Qader, Afsheen Aman,