کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8327559 | 1540201 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Structure and conformation of α-glucan extracted from Agaricus blazei Murill by high-speed shearing homogenization
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Structure and conformation of α-glucan extracted from Agaricus blazei Murill by high-speed shearing homogenization Structure and conformation of α-glucan extracted from Agaricus blazei Murill by high-speed shearing homogenization](/preview/png/8327559.png)
چکیده انگلیسی
Agaricus blazei Murill is an edible and medicinal mushroom favored in many countries, by virtue of both its delicious taste and its potential health benefits such as its purported anticancer activity. A neutral α-glucan (ABM40-1) with a carbohydrate content of 96% was purified from the high-speed shearing homogenization extracts of A. Blazei Murill by ethanol precipitation and column chromatography. Methylation analysis along with nuclear magnetic resonance spectroscopy revealed that ABM40-1 was an α-(1 â 4)-d-glucopyranan with O-6 position occasionally occupied with α-Glcp-(1 â or α-Glcp-(1 â 6)-β-Glcp-(1 â side chains. A weight-average molecular weight of 7.34 Ã 106 Da was determined for ABM40-1 and its chain in solution was revealed as a compact sphere by size exclusion chromatography (SEC) coupled with a laser light scattering. This spherical conformation was also further confirmed by Congo red test and using atom force microscopy. These results suggest it would be worthwhile to further study the potential bioactivities of ABM40-1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 113, 1 July 2018, Pages 558-564
Journal: International Journal of Biological Macromolecules - Volume 113, 1 July 2018, Pages 558-564
نویسندگان
Anqiang Zhang, Jiaying Deng, Xiaoqing Liu, Pengfei He, Liang He, Fuming Zhang, Robert J. Linhardt, Peilong Sun,