کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8327603 | 1540201 | 2018 | 22 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transgenic tobacco plants expressing a P1B-ATPase gene from Populus tomentosa Carr. (PtoHMA5) demonstrate improved cadmium transport
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Heavy metal ATPase (HMA) plays an important role in phytoremediation via long-distance transportation from root to shoot. In this report, we identified a heavy metal ATPase gene, PtoHMA5, from Populus tomentosa Carr. Its encoded peptide consists of 967 amino acids and has eight trans-membrane motifs inside. Tobacco plants were transformed with this gene via Agrobacterium tumefaciens-mediated method. After exposure to 50Â mg/LCdCl2 for 10Â d, the transgenic lines displayed higher cadmium accumulation in leaves than did the wild-type plants with an absolute increase of 25.04%, while the transfer coefficient increased by 16.01%-43.25%. Physiological testing including assessment of relative electrolytic leakage (REL), malondialdehyde (MDA) content, and chlorophyll content revealed that the transgenic lines were seriously affected when compared with the wild-type plants. In summary, PtoHMA5 is really involved in cadmium transport from root to shoot but is not associated with the removal of cadmium toxicity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 113, 1 July 2018, Pages 655-661
Journal: International Journal of Biological Macromolecules - Volume 113, 1 July 2018, Pages 655-661
نویسندگان
Xiaotong Wang, Junkai Zhi, Xinru Liu, Hao Zhang, Huabo Liu, Jichen Xu,