کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8327830 | 1540203 | 2018 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Total fractionation and characterization of the water-soluble polysaccharides isolated from Enteromorpha intestinalis
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Water-soluble crude polysaccharides (WE) were extracted from the Enteromorpha intestinalis with hot water and further fractionated on a DEAE-52 Cellulose chromatography column and Sepharose CL-6B gel-permeation chromatography to afford one neutral fraction (WE-11) and five acidic fractions (WE-21, WE-31, WE-32, WE-41 and WE-42). Monosaccharide analysis showed that WE-32, WE-41 and WE-42 were all composed of Rha and GlcA, WE-21 and WE-31 contained Man and Rha, and WE-11 was composed of GlcA and Xyl. The molecular weight of WE-11, WE-21, WE-31 WE-32, WE-41 and WE-42 were 2.23â¯Ãâ¯105, 1.42â¯Ãâ¯105, 1.42â¯Ãâ¯105, 2.62â¯Ãâ¯104, 2.23â¯Ãâ¯105 and 8.09â¯Ãâ¯104, respectively. Then, antioxidant activities in vitro were investigated on the basis of DPPH radical assay, reducing power assay, hydroxyl radical assay and superoxide radical assay. Of all polysaccharides fractions, WE-32 and WE-41 had relative higher content of sulfate and GlcA. In addition, WE-32 and WE-41 showed relative stronger antioxidant activity and inhibitory activity in vitro. The antioxidant activities of polysaccharides were not a function of a single factor but a combination of several factors, such as monosaccharide composition, molecular weights, protein content, uronic acid and sulfate content.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 111, May 2018, Pages 319-325
Journal: International Journal of Biological Macromolecules - Volume 111, May 2018, Pages 319-325
نویسندگان
Xia Li, Feng Xiong, Yufeng Liu, Feifei Liu, Zaibin Hao, Huiying Chen,