Multi-step synthesis, spectroscopic studies of biological active steroidal thiosemicarbazones and their palladium (II) complex as macromolecules

Steroidal Pd (II) metal complexes were synthesized by the reaction of steroidal thiosemicarbazones with [Pd (DMSO)2 Cl2]. The steroidal thiosemicarbazones coordinate to palladium through the azomethine nitrogen and thionic sulfur. The thiosemicarbazone derivatives were obtained by the reaction of thiosemicarbazide with steroidal ketones. The identity of steroidal thiosemicarbazones and their Pd(II) metal complexes were elucidated by IR, 1H, 13C NMR, FAB mass spectroscopic methods, elemental analysis and TGA analysis. The antibacterial activity of these compounds were tested in vitro by disk diffusion assay against two Gram-positive and two Gram-negative bacteria. The results showed that the Pd(II) complexes are better growth inhibitors than steroidal thiosemicarbazones of both types of the bacteria (gram-positive and gram-negative). The compound 2a {[Pd(C35H54N3SCl)Cl2]} is a as good as antibacterial agent as compared to amoxicillin.