A transparent wound dressing based on bacterial cellulose whisker and poly(2-hydroxyethyl methacrylate)

The current study was aimed to develop a transparent wound dressing comprised of bacterial cellulose (BC) and poly (2-hydroxyethyl methacrylate) (PHEMA) hydrogel coated with silver (Ag) nanoparticles. Briefly, different concentrations of BC whiskers (BCWs) were added into the HEMA solution to form PHEMA/BCWs hydrogel with volume ratio of monomer HEMA and BCWs as 7:3 and 1:1. The addition of BCWs into PHEMA matrix improved its equilibrium water content and light transmittance about 20%-40% and 10%, respectively. The Young's modulus for PHEMA was found to be 0.72 MPa, which was improved to 0.57 MPa and 0.50 MPa for PHEMA/BCWs 7:3 and PHEMA/BCWs 1:1, respectively. Further, immersion of PHEMA/BCWs hydrogel in the AgNO3 and NaBH4 solutions bestowed it with antibacterial property and produced inhibition zones of 0.5 ± 0.15 cm and 0.25 ± 0.15 cm against Escherichia coli and Staphylococcus aureus, respectively. Similarly, PHEMA/BCWs prepared with 0.001 M AgNO3 and 0.001 M NaBH4 solutions showed 99% and 90% reduction in colony forming unit (CFU) for E. coli and S. aureus, respectively, after 24 h. The PHEMA/BCWs/Ag hydrogel facilitated the growth of NIH3T3 fibroblast, showing their low toxicity. These results demonstrate the suitability of PHEMA/BCWs/Ag hydrogel for its application as potential transparent wound dressing material for skin repair.