کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8329934 | 1540239 | 2015 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Kinetic and thermodynamic studies of a novel acid protease from Aspergillus foetidus
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Kinetic and thermodynamic studies of a novel acid protease from Aspergillus foetidus Kinetic and thermodynamic studies of a novel acid protease from Aspergillus foetidus](/preview/png/8329934.png)
چکیده انگلیسی
The kinetics of a thermostable extracellular acid protease produced by an Aspergillus foetidus strain was investigated at different pH, temperatures and substrate concentrations. The enzyme exhibited maximal activity at pH 5.0 and 55 °C, and its irreversible deactivation was well described by first-order kinetics. When temperature was raised from 55 to 70 °C, the deactivation rate constant increased from 0.018 to 5.06 hâ1, while the half-life decreased from 37.6 to 0.13 h. The results of activity collected at different temperatures were then used to estimate, the activation energy of the hydrolysis reaction (E* = 19.03 kJ/mol) and the standard enthalpy variation of reversible enzyme unfolding (ÎH°U = 19.03 kJ/mol). The results of residual activity tests carried out in the temperature range 55-70 °C allowed estimating the activation energy (E*d = 314.12 kJ/mol), enthalpy (311.27 â¤Â (ÎH°d â¤Â 311.39 kJ/mol), entropy (599.59 â¤Â ÎS*d â¤Â 610.49 kJ/mol K) and Gibbs free energy (103.18 â¤Â ÎG*d â¤Â 113.87 kJ/mol) of the enzyme irreversible denaturation. These thermodynamic parameters suggest that this new protease is highly thermostable and could be important for industrial applications. To the best of our knowledge, this is the first report on thermodynamic parameters of an acid protease produced by A. foetidus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 81, November 2015, Pages 17-21
Journal: International Journal of Biological Macromolecules - Volume 81, November 2015, Pages 17-21
نویسندگان
Paula Monteiro Souza, Bahar Aliakbarian, Edivaldo Ximenes Ferreira Filho, Pérola Oliveira Magalhães, Adalberto Pessoa Junior, Attilio Converti, Patrizia Perego,