کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8330087 | 1540239 | 2015 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Protein secondary structure of Green Lynx spider dragline silk investigated by solid-state NMR and X-ray diffraction
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Protein secondary structure of Green Lynx spider dragline silk investigated by solid-state NMR and X-ray diffraction Protein secondary structure of Green Lynx spider dragline silk investigated by solid-state NMR and X-ray diffraction](/preview/png/8330087.png)
چکیده انگلیسی
In this study, the secondary structure of the major ampullate silk from Peucetia viridans (Green Lynx) spiders is characterized by X-ray diffraction and solid-state NMR spectroscopy. From X-ray diffraction measurement, β-sheet nanocrystallites were observed and found to be highly oriented along the fiber axis, with an orientational order, fc â 0.98. The size of the nanocrystallites was determined to be on average 2.5 nm Ã 3.3 nm Ã 3.8 nm. Besides a prominent nanocrystalline region, a partially oriented amorphous region was also observed with an fa â 0.89. Two-dimensional 13C-13C through-space and through-bond solid-state NMR experiments were employed to elucidate structure details of P. viridans silk proteins. It reveals that β-sheet nanocrystallites constitutes 40.0 ± 1.2% of the protein and are dominated by alanine-rich repetitive motifs. Furthermore, based upon the NMR data, 18 ± 1% of alanine, 60 ± 2% glycine and 54 ± 2% serine are incorporated into helical conformations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 81, November 2015, Pages 171-179
Journal: International Journal of Biological Macromolecules - Volume 81, November 2015, Pages 171-179
نویسندگان
Dian Xu, Xiangyan Shi, Forrest Thompson, Warner S. Weber, Qiushi Mou, Jeffery L. Yarger,