کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8330241 1540238 2016 16 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Beta-chitosan extracted from Loligo Japonica for a potential use to inhibit Newcastle disease
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Beta-chitosan extracted from Loligo Japonica for a potential use to inhibit Newcastle disease
چکیده انگلیسی
Beta-chitosan has a parallel structure, which differs from alpha-chitosan's antiparallel structure while producing different properties and difficulties. In this paper, we prepared the beta-chitosan through acid and alkali methods and the resultant material was characterized by elemental analysis, FT-IR, HPLC, XRD, NMR and AFS. To increase the solubility and biological activity of the beta-chitosan, we degraded it through microwave-assisted process. After characterization, we determined that the chitosan had not changed its configuration during the reaction with H2O2 under microwave irradiation. The inhibitory activity of the degraded chitosan for Newcastle disease was revealed by a hemagglutination test and RT-PCR. The yield of the beta-chitosan was approximately 30%, and its molecular weight can be degraded to 1000 to 10,000 g/mol. Moreover, the degraded β-chitosan has higher antiviral activity, reducing the hemagglutination titre to zero, compared with alpha-chitosan. Therefore, beta-chitosan has good development prospects during the development of veterinary drugs for Newcastle disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 82, January 2016, Pages 614-620
نویسندگان
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