کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8330330 | 1540238 | 2016 | 20 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Interaction of a snake venom l-amino acid oxidase with different cell types membrane
ترجمه فارسی عنوان
اثر متقابل اکسیداز سم مار-اسید آمینه با غشای سلولی مختلف سلولی
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کلمات کلیدی
FCSinitial surface pressureπiπc3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromideCFUNATFITCLPGPBMCl-amino acid oxidase - L-آمینو اسید اکسیدازMTT - MTTImmobilized enzyme - آنزیم ImmobilizedMembrane interaction - تعامل غشاءfetal calf serum - سرم گوساله جنینPeripheral blood mononuclear cell - سلول تک هسته ای خون محیطیcolony-forming unit - واحد تشکیل کلنیPhospholipid monolayers - یکپارچه فسفولیپید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Snake venom l-amino acid oxidases are multifunctional enzymes that exhibited a wide range of pharmacological activities. Although it has been established that these activities are primarily caused by the H2O2 generated in the enzymatic reaction, the molecular mechanism, however, has not been fully investigated. In this work, LAAO interaction with cytoplasmic membranes using different cell types and Langmuir interfacial monolayers was evaluated. The Cerastes cerastes venom LAAO (CC-LAAO) did not exhibit cytotoxic activities against erythrocytes and peripheral blood mononuclear cells (PBMC). However, CC-LAAO caused cytotoxicity on several cancer cell lines and induced platelet aggregation in dose-dependent manner. Furthermore, the enzyme showed remarkable effect against Gram-positive and Gram-negative bacteria. These activities were inhibited on the addition of catalase or substrate analogs, suggesting that H2O2 liberationà is required for these effects. Binding studies revealed that CC-LAAO binds to the cell surface and enables the production of highly localized concentration of H2O2 in or near the binding interfaces. On another hand, the interaction of CC-LAAO with a mimetic phospholipid film was evaluated, for the first time, using a monomolecular film technique. Results indicated that phospholipid/CC-LAAO interactions are not involved in their binding to membrane and in their pharmacological activities.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 82, January 2016, Pages 757-764
Journal: International Journal of Biological Macromolecules - Volume 82, January 2016, Pages 757-764
نویسندگان
Zaineb Abdelkafi-Koubaa, Imen Aissa, Maram Morjen, Nadia Kharrat, Mohamed El Ayeb, Youssef Gargouri, Najet Srairi-Abid, Naziha Marrakchi,