PEG-based ultrasound-assisted enzymatic extraction of polysaccharides from Ginkgo biloba leaves

In this study, one kind of environmentally friendly solvents named polyethylene glycol (PEG) was developed for the ultrasound-assisted enzymatic extraction (UAEE) of polysaccharides from Gingko biloba leaves (GBLP). Box-Behnken design (BBD) was used to optimize the UAEE conditions of GBLP. Results showed that the optimal extraction conditions were: a pH of 4.34, an extraction temperature of 51.88 °C and an extraction time of 37.13 min. Under these optimal extraction conditions, the GBLP yield was 7.29 ± 0.21%, which was well in agreement with the value predicted by the mathematical model.