کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8331287 | 1540241 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation, characterization, interaction of a thiazolekinase (Plasmodium falciparum) with silver nanoparticles
ترجمه فارسی عنوان
جداسازی، مشخص کردن، تعامل یک تیازولیناز (پلاسمودیوم فالسیپاروم) با نانوذرات نقره
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Malaria, a mosquito-borne infectious disease, is caused by the Plasmodium genus, and remains one of the greatest health challenges worldwide. The malarial parasite possess a biosynthetic pathway for the B-group vitamin incorporating the thiamine metabolizing enzymes; humans on the other hand cannot synthesize the vitamin and require it from within their diet. The vitamin B1 biosynthetic enzyme 5-(2-hydroxyethyl)-4-methylthioazolekinase [EC. 2.7.1.50] from Plasmodium (PfThzK) is particularly attractive as a biomedical target since any inhibition of this enzyme may lead to an effective treatment for malaria. In the present study, PfThzK was recombinantly produced as a 6à His fusion protein in Escherichia coli BL21(DE3) and purified using nickel affinity and size exclusion chromatography. The enzyme was monomeric with a molecular mass of 34 kDa, a specific activity of 295.04 nmol minâ1 mgâ1 and showed an optimum temperature and pH of 37 °C and 7.5, respectively. The purified PfThzK was non-competitively inhibited (79%) by silver nanoparticles (2-6 nm); Ki = 6.45 μM. A mechanism is suggested for the interaction of the silver nanoparticle with PfThzK through two sulphur bearing amino acids (Met1, Cys206) on the surface of each subunit of the enzyme.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 79, August 2015, Pages 644-653
Journal: International Journal of Biological Macromolecules - Volume 79, August 2015, Pages 644-653
نویسندگان
J. Yao, J. van Marwijk, B. Wilhelmi, C.G. Whiteley,