کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8331402 | 1540242 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Structural identification and immunostimulating activity of a Laminaria japonica polysaccharide
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Structural identification and immunostimulating activity of a Laminaria japonica polysaccharide Structural identification and immunostimulating activity of a Laminaria japonica polysaccharide](/preview/png/8331402.png)
چکیده انگلیسی
In the present study, a new water-soluble polysaccharide (LJP-11) was obtained from Laminaria japonica by anion exchange DEAE-cellulose chromatography and Sephacryl S-500 chromatography. The average molecular weight of this polysaccharide was estimated to be about 2.89 Ã 106 Da by high performance liquid chromatography system. Gas chromatography showed that LJP-11 was composed of arabinose, mannose and glucose in a molar ratio of 1.0:1.16:6.33. LJP-11 contains a long backbone consisting of (1â4)-β-d-GlcpAc, (1â4)-α-d-Glcp, (1â6)-β-d-Glcp and (1â3,6)-α-d-Manp. The 1-linked β-l-Araf was linked to the C-6 of (1â3)-α-d-Manp and the sulfate group was attached to the C-4 of (1â6)-β-d-Glcp. Pharmacological tests displayed that LJP-11 can stimulate macrophages to release NO, IL-6, TNF-α and IL-10 as well as the up-regulation of their gene expressions, indicating LJP-11 has beneficial effects on immunostimulation. Moreover, LJP-11 exhibited positive effects on the translocation of NF-κB p65 from cytoplasm to nucleus and the phosphorylation of IκBα, ERK1/2, JNK1/2 and P38 in macrophages. These results suggested that the activation of MAPK and NF-κB signaling pathways is one of the mechanisms responsible for the immunostimulating activity of LJP-11.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 78, July 2015, Pages 429-438
Journal: International Journal of Biological Macromolecules - Volume 78, July 2015, Pages 429-438
نویسندگان
Xue-Qiang Zha, Chao-Qun Lu, Shao-Hua Cui, Li-Hua Pan, Hai-Lin Zhang, Jun-Hui Wang, Jian-Ping Luo,