کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8331515 | 1540245 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Keratinous waste decomposition and peptide production by keratinase from Geobacillus stearothermophilus AD-11
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Keratinous waste decomposition and peptide production by keratinase from Geobacillus stearothermophilus AD-11 Keratinous waste decomposition and peptide production by keratinase from Geobacillus stearothermophilus AD-11](/preview/png/8331515.png)
چکیده انگلیسی
A keratinolytic proteinase was cloned from thermophilic bacterium Geobacillus stearothermophilus AD-11 and was expressed in Escherichia coli BL21(DE3). Recombinant keratinolytic proteinase (RecGEOker) with an estimated molecular weight of 57 kDa was purified and keratinase activity was measured. RecGEOker showed optimal activity at pH 9 and 60 °C. Recombinant keratinolytic proteinase showed the highest substrate specificity toward keratin from wool > collagen > sodium caseinate > gelatin > and BSA in descending order. RecGEOker is applicable for efficient keratin waste biodegradation and can replace conventional non-biological hydrolysis processes. High-value small peptides obtained from enzymatic biodegradation by RecGEOker are suitable for industrial application in white and/or green biotechnology for use as major additives in various products.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 75, April 2015, Pages 158-165
Journal: International Journal of Biological Macromolecules - Volume 75, April 2015, Pages 158-165
نویسندگان
Audrius Gegeckas, Renata GudiukaitÄ, Janusz Debski, Donaldas Citavicius,