کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8332710 | 1540248 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Breast anticancer drug tamoxifen and its metabolites bind tRNA at multiple sites
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
The binding sites of breast anticancer drug tamoxifen and its metabolites with tRNA were located by FTIR, CD, UV-visible, and fluorescence spectroscopic methods and molecular modeling. Structural analysis showed that tamoxifen and its metabolites bind tRNA at several binding sites with overall binding constants of Ktam-tRNA = 5.2 (±0.6) Ã 104 Mâ1, K4-hydroxytam-tRNA = 6.5 (±0.5) Ã 104 Mâ1 and Kendox-tRNA = 1.3 (±0.2) Ã 104 Mâ1. The number of binding sites occupied by drug molecules on tRNA were 1 (tamoxifen), 0.8 (4-hydroxitamoxifen) and 1.2 (endoxifen). Docking showed the participation of several nucleobases in drug-tRNA complexes with the free binding energy of â4.31 (tamoxifen), â4.45 (4-hydroxtamoxifen) and â4.38 kcal/mol (endoxifen). The order of binding is 4-hydroxy-tamoxifen > tamoxifen > endoxifen. Drug binding did not alter tRNA conformation from A-family structure, while biopolymer aggregation occurred at high drug concentration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 72, January 2015, Pages 692-698
Journal: International Journal of Biological Macromolecules - Volume 72, January 2015, Pages 692-698
نویسندگان
P. Bourassa, T.J. Thomas, J. Bariyanga, H.A. Tajmir-Riahi,