کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8333170 | 1540253 | 2014 | 31 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Metal ion activated lipase from halotolerant Bacillus sp. VITL8 displays broader operational range
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Metal ion activated lipase from halotolerant Bacillus sp. VITL8 displays broader operational range Metal ion activated lipase from halotolerant Bacillus sp. VITL8 displays broader operational range](/preview/png/8333170.png)
چکیده انگلیسی
Lipase producing halo tolerant Bacillus sp. VITL8 was isolated from oil contaminated areas of Vellore. The identity of the organism was established by 16S rDNA sequence, in addition to the morphological and biochemical characterization. The purified enzyme (22 kDa, 8680 U/mg) exhibited optimal activity at pH 7.0 and 40 °C and retained more than 50% of its activity in the NaCl concentration range of 0-3.0 M, pH 6.0-10.0 and 10-60 °C. Secondary structure analysis, using circular dichroism, revealed that the enzyme is composed of 38% α-helix and 29% β-turns. The lipase activity significantly increased in the presence of (1 mM) Mn2+ (139%), Ca2+ (134%) and Mg2+ (130%). Organic solvents such as butanol and acetonitrile (25%, v/v) enhanced the activity whereas DMSO (25% v/v) retained the activity. The Km of enzyme-p-Nitrophenyl palmitate complex was determined to be 191 μM with a Vmax of 68 μM/mg/min. Though halotolerant Bacillus sp. has been explored for hydrocarbon degradation, to our knowledge this is the first report on the lipase activity of the isolate. The characteristics of the enzyme presented in this report, imply broader operational range of the enzyme and therefore could be suitable for many of the industrial chemical processes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 67, June 2014, Pages 380-386
Journal: International Journal of Biological Macromolecules - Volume 67, June 2014, Pages 380-386
نویسندگان
Lavanya Balaji, Gurunathan Jayaraman,