کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8333281 | 1540260 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Antitumor activity of polysaccharides from Lepista sordida against laryngocarcinoma in vitro and in vivo
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Antitumor activity of polysaccharides from Lepista sordida against laryngocarcinoma in vitro and in vivo Antitumor activity of polysaccharides from Lepista sordida against laryngocarcinoma in vitro and in vivo](/preview/png/8333281.png)
چکیده انگلیسی
Four water-soluble polysaccharides (LSPa1, LSPb1, LSPb2 and LSPc1) extracted from the fruiting bodies of Lepista sordida were prepared by DEAE cellulose-52 anion-exchange and Sepharose 6 Fast Flow gel-permeation chromatography. LSPa1 was composed of Glc, Man and Gal with a molar ratio of 3.2: 1.3: 0.6. LSPb1, LSPb2 and LSPc1 were composed of Glc, Man, Gal, Rha and Ara in the proportions of 2.6: 2.1: 0.9: 0.6: 0.3, 1.2: 1.0: 2.3: 0.6: 0.5, 0.2: 0.3: 2.3: 0.3: 2.0, respectively. The high-performance gel-permeation chromatography (HPGPC) analysis showed that the average molecular weight (Mw) of four polysaccharides were approximately 156, 134, 96 and 57Â kDa, respectively. MTT and lactate dehydrogenase (LDH) assays indicated that three acidic polysaccharides, namely LSPb1, LSPb2 and LSPc1, possessed more potent antiproliferative effect on human laryngocarcinoma Hep-2 cells than neutral polysaccharide LSPa1 in a dose- and time-dependent manner and the effect increased in the order of LSPa1Â <Â LSPb2Â <Â LSPb1Â <Â LSPc1, which is the same as their uronic acid content. Furthermore the tumor volume and tumor weight were also obviously suppressed after two weeks' treatment of LSPc1 to mice engrafted with Hep-2 cancer cells. Results of these studies demonstrated that the polysaccharide LSPc1 had a potential application as natural antitumor drugs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 60, September 2013, Pages 235-240
Journal: International Journal of Biological Macromolecules - Volume 60, September 2013, Pages 235-240
نویسندگان
Susheng Miao, Xionghui Mao, Rong Pei, Suping Miao, Cheng Xiang, Yuanjing Lv, Xianguang Yang, Ji Sun, Shenshan Jia, Yvpeng Liu,