کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8333329 | 1540260 | 2013 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of osmolytes on Pelodiscus sinensis creatine kinase: A study on thermal denaturation and aggregation
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کلمات کلیدی
Pelodiscus sinensis1-anilinonaphthalene-8-sulfonate - 1-anilinonphthalene-8-sulfonateThermal denaturation - denaturation حرارتیDMSO - DMSOLC–MS/MS - LC-MS / MSAdenosine Triphosphate - آدنوزین تری فسفاتATP - آدنوزین تری فسفات یا ATPOsmolyte - اسمولیتDimethyl sulfoxide - دیمتیل سولفواکسیدANS - سالLiquid chromatography-tandem mass spectrometry - طیف سنجی جرمی کروماتوگرافی مایع دو طرفهCreatine kinase - کراتین کیناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
The protective effect of osmolytes on the thermal denaturation and aggregation of Pelodiscus sinensis muscle creatine kinase (PSCK) was investigated by a combination of spectroscopic methods and thermodynamic analysis. Our results demonstrated that the addition of osmolytes, such as glycine and proline, could prevent thermal denaturation and aggregation of PSCK in a concentration-dependent manner. When the concentration of glycine and proline increased in the denatured system, the relative activation was significantly enhanced; meanwhile, the aggregation of PSCK during thermal denaturation was decreased. Spectrofluorometer results showed that glycine and proline significantly decreased the tertiary structural changes of PSCK and that thermal denaturation directly induced PSCK aggregation. In addition, we also built the 3D structure of PSCK and osmolytes by homology models. The results of computational docking simulations showed that the docking energy was relatively low and that the clustering groups were spread to the surface of PSCK, indicating that osmolytes directly protect the surface of the protein. P. sinensis are poikilothermic and quite sensitive to the change of ambient temperature; however, there were few studies on the thermal denaturation of reptile CK. Our study provides important insight into the protective effects of osmolytes on thermal denaturation and aggregation of PSCK.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 60, September 2013, Pages 277-287
Journal: International Journal of Biological Macromolecules - Volume 60, September 2013, Pages 277-287
نویسندگان
Wei Wang, Jinhyuk Lee, Qin-Xin Jin, Nai-Yun Fang, Yue-Xiu Si, Shang-Jun Yin, Guo-Ying Qian, Yong-Doo Park,