کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8333548 | 1540261 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Preparation and evaluation of lysozyme-loaded nanoparticles coated with poly-γ-glutamic acid and chitosan
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Preparation and evaluation of lysozyme-loaded nanoparticles coated with poly-γ-glutamic acid and chitosan Preparation and evaluation of lysozyme-loaded nanoparticles coated with poly-γ-glutamic acid and chitosan](/preview/png/8333548.png)
چکیده انگلیسی
To improve the application of lysozymes, methods for coating lysozymes with poly-γ-glutamic acid and chitosan were studied. Several lysozyme-loaded chitosan/poly-γ-glutamic acid composite nanosystems for loading and controlling the release of lysozymes were established. The lysozyme loading content and efficiency of the different systems were examined. The antibacterial activity of the composite nanoparticles was also investigated. Results showed that when the lysozymes were coated with poly-γ-glutamic acid and further rewrapped with chitosan, smooth spherical composite nanoparticles were obtained; the loading efficiency and loading content reached 76% and 40%, respectively. The lysozyme release in vitro was slow and presented a two-stage programmed release. Antibacterial testing in vitro indicated that lysozyme-loaded nanoparticles coated with poly-γ-glutamic acid/chitosan had outstanding antibacterial activity. An obvious assembly of bacterial cells and composite nanoparticles was observed during co-incubation. Therefore, the poly-γ-glutamic acid/chitosan composite coating broadened the antibacterial spectrum of the composite lysozyme nanoreagent, and presented satisfactory antibacterial effect. The lysozyme-loaded chitosan/poly-γ-glutamic acid nanocoating system established in this research could provide reference for coating and controlled releasing of alkaline proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 59, August 2013, Pages 201-207
Journal: International Journal of Biological Macromolecules - Volume 59, August 2013, Pages 201-207
نویسندگان
Yong Liu, Yan Sun, Yaoxing Xu, Hai Feng, Sida Fu, Jiangwu Tang, Wei Liu, Dongchang Sun, Hua Jiang, Shaochun Xu,