کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8333865 | 1540263 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immunostimulatory activity and structure of polysaccharide from Streptococcus equi subsp. zooepidemicus
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Immunostimulatory activity and structure of polysaccharide from Streptococcus equi subsp. zooepidemicus Immunostimulatory activity and structure of polysaccharide from Streptococcus equi subsp. zooepidemicus](/preview/png/8333865.png)
چکیده انگلیسی
In the present study, crude capsule polysaccharides from Streptococcus equi subsp. zooepidemicus C55129 (CP) were purified by DEAE cellulose-52 column and Sephadex G-100 column chromatography to afford purified fractions of CP-1, CP-2 and CP-3. The relative molecular weights of CP-1, CP-2 and CP-3 were 29.0, 891.0 and 1338.0 kDa, respectively. CP-1 was composed of mannose, glucose, arabinose and galactose in a percentage ratio of 66.15:28.97:2.43:2.45. CP-2 was composed of mannose, glucose, galactose and glucuronic acid in a percentage ratio of 40.94:27.71:5.96:25.39. In CP-1, there were pyranose rings, α-configuration glycosidic bond residues, one backbone chain (1â6 glycosidic bonds) and two branch chains (1â3 and 1â4 glycosidic bonds) in one repeating unit. In vitro immunostimulatory assay, it was found that CP could promote the splenocyte proliferation and increase the activity of acid phosphatase in peritoneal macrophages. The immunostimulatory activity of CP-1 might be related to its monosaccharide composition, molecular weight and α-configuration glycosidic bond.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 57, June 2013, Pages 218-225
Journal: International Journal of Biological Macromolecules - Volume 57, June 2013, Pages 218-225
نویسندگان
Chunlin Ke, Deliang Qiao, Jianguang Luo, Zuomei Li, Yi Sun, Hong Ye, Xiaoxiong Zeng,