کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8334112 | 1540270 | 2012 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Structural analysis of Herbaspirillum seropedicae lipid-A and of two mutants defective to colonize maize roots
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Structural analysis of Herbaspirillum seropedicae lipid-A and of two mutants defective to colonize maize roots Structural analysis of Herbaspirillum seropedicae lipid-A and of two mutants defective to colonize maize roots](/preview/png/8334112.png)
چکیده انگلیسی
Lipid-A was isolated by mild acid hydrolysis from lipopolysaccharides extracted from cells of Herbaspirillum seropedicae, strain SMR1, and from two mutants deficient in the biosynthesis of rhamnose (rmlBâ and rmlCâ). Structural analyzes were carried out using MALDI-TOF and derivatization by per-O-trimethylsilylation followed by GC-MS in order to determine monosaccharide and fatty acid composition. De-O-acylation was also performed to determine the presence of N-linked fatty acids. Lipid-A from H. seropedicae SMR1 showed a major structure comprising 2-amino-2-deoxy-glucopyranose-(1 â 6)-2-amino-2-deoxy-glucopyranose phosphorylated at C4â² and C1 positions, each carrying a unit of 4-amino-4-deoxy-arabinose. C2 and C2â² positions were substituted by amide-linked 3-hydroxy-dodecanoic acids. Both rhamnose-defective mutants showed similar structure for their lipid-A moieties, except for the lack of 4-amino-4-deoxy-arabinose units attached to phosphoryl groups.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 51, Issue 4, November 2012, Pages 384-391
Journal: International Journal of Biological Macromolecules - Volume 51, Issue 4, November 2012, Pages 384-391
نویسندگان
Rodrigo V. Serrato, Eduardo Balsanelli, Guilherme L. Sassaki, Russell W. Carlson, Artur Muszynski, Rose A. Monteiro, Fábio O. Pedrosa, Emanuel M. Souza, Marcello Iacomini,