Purification, physico-chemical and kinetic properties of the deglycosylated Talaromyces thermophilus lipase

► An appearance of a second shorter form of Talaromyces thermophilus lipase. ► This form was purified to homogeneity and its N-terminal sequence was identified. ► The glycan chain content of this form confirmed its deglycosylation at Asn 33. ► N-glycans are determinant for its optimal catalytic activity and thermal stability.