کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8335034 1540277 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Synthesis and anticoagulant activity of the quaternary ammonium chitosan sulfates
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Synthesis and anticoagulant activity of the quaternary ammonium chitosan sulfates
چکیده انگلیسی
Quaternary ammonium chitosan sulfates with diverse degrees of substitution (DS) ascribed to sulfate groups between 0.52 and 1.55 were synthesized by reacting quaternary ammonium chitosan with an uncommon sulfating agent (N(SO3Na)3) that was prepared from sodium bisulfite (NaHSO3) through reaction with sodium nitrite (NaNO2) in the aqueous system homogeneous. The structures of the derivatives were characterized by FTIR, 1H NMR and 13C NMR. The factors affecting DS of quaternary ammonium chitosan sulfates which included the molar ratio of NaNO2 to quaternary ammonium chitosan, sulfated temperature, sulfated time and pH of sulfated reaction solution were investigated in detail. Its anticoagulation activity in vitro was determined by an activated partial thromboplastin time (APTT) assay, a thrombin time (TT) assay and a prothrombin time (PT) assay. Results of anticoagulation assays showed quaternary ammonium chitosan sulfates significantly prolonged APTT and TT, but not PT, and demonstrated that the introduction of sulfate groups into the quaternary ammonium chitosan structure improved its anticoagulant activity obviously. The study showed its anticoagulant properties strongly depended on its DS, concentration and molecular weight.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 50, Issue 1, 1 January 2012, Pages 31-37
نویسندگان
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