Optimization on preparation condition of epimedium polysaccharide liposome and evaluation of its adjuvant activity

► The immunoehancement of epimedium polysaccharide liposome is superior to epimedium polysaccharide or liposome alone. ► The high dose of epimedium polysaccharide liposome possessed the best efficacy. ► The optimal preparation condition of epimedium polysaccharide liposome was selected with an orthogonal L9 (34) test design.