کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8336098 | 1540431 | 2018 | 19 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differentiation of neural crest stem cells from nasal mucosa into motor neuron-like cells
ترجمه فارسی عنوان
تمایز سلول های بنیادی خلط عصبی از مخاطی بینی به سلول های سلول های عصبی موتور
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کلمات کلیدی
تفکیک، سلول های عصبی موتور، سلول های بنیادی اکتومسنشیمال رژیم غذایی، آسیب نخاعی،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Cell transplantation is a potential therapeutic approach for repairing neuropathological and neurodegenerative disorders of central nervous system by replacing the degenerated cells with new ones. Among a variety of stem cell candidates to provide these new cells, olfactory ectomesenchymal stem cells (OE-MSCs) have attracted a great attention due to their neural crest origin, easy harvest, high proliferation, and autologous transplantation. Since there is no report on differentiation potential of these cells into motor neuron-like cells, we evaluated this potential using Real-time PCR, flowcytometry and immunocytochemistry after the treatment with differentiation cocktail containing retinoic acid and Sonic Hedgehog. Immunocytochemistry staining of the isolated OE-MSCs demonstrated their capability to express nestin and vimentin, as the two markers of primitive neuroectoderm. The motor neuron differentiation of OE-MSCs resulted in changing their morphology into bipolar cells with high expression of motor neuron markers of ChAT, Hb-9 and Islet-1 at the level of mRNA and protein. Consequently, we believe that the OE-MSCs have great potential to differentiate into motor neuron-like cells and can be an ideal stem cell source for the treatment of motor neuron-related disorders of central nervous system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chemical Neuroanatomy - Volume 92, October 2018, Pages 35-40
Journal: Journal of Chemical Neuroanatomy - Volume 92, October 2018, Pages 35-40
نویسندگان
Zohreh Bagher, Seyed Kamran Kamrava, Rafieh Alizadeh, Mohammad Farhadi, Moloud Absalan, Masoumeh Falah, Faezeh Faghihi, Arash Zare-Sadeghi, Ali Komeili,