کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8336109 | 1540431 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Down-regulation of fatty acid binding protein 7 (Fabp7) is a hallmark of the postpartum brain
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کلمات کلیدی
qPCRFABP7MPOAVTAMPFCLSVLSDGFAPLSIGene expression - بیان ژنlateral septum - سپتوم جانبیmedial prefrontal cortex - قشر غده پروسترولPVN - مالیات بر ارزش افزودهmedial preoptic area - منطقه پیشوپتیک مدیاMouse - موشventral tegmental area - ناحیه تگمنتوم شکمیknockout - ناکاوتwild-type - نوع وحشیNucleus accumbens - هسته accumbensparaventricular nucleus - هسته پروژسترویکlateral hypothalamus - هیپوتالاموس جانبیquantitative real-time polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی استGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایال
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Down-regulation of fatty acid binding protein 7 (Fabp7) is a hallmark of the postpartum brain Down-regulation of fatty acid binding protein 7 (Fabp7) is a hallmark of the postpartum brain](/preview/png/8336109.png)
چکیده انگلیسی
Fatty acid binding protein 7 (Fabp7) is a versatile protein that is linked to glial differentiation and proliferation, neurogenesis, and multiple mental health disorders. Recent microarray studies identified a robust decrease in Fabp7 expression in key brain regions of the postpartum rodents. Given its diverse functions, Fabp7 could play a critical role in sculpting the maternal brain and promoting the maternal phenotype. The present study aimed at investigating the expression profile of Fabp7 across the postpartum CNS. Quantitative real-time PCR (qPCR) analysis showed that Fabp7 mRNA was consistently down-regulated across the postpartum brain. Of the 9 maternal care-related regions tested, seven exhibited significant decreases in Fabp7 in postpartum (relative to virgin) females, including medial prefrontal cortex (mPFC), nucleus accumbens (NA), lateral septum (LS), bed nucleus of stria terminalis dorsal (BnSTd), paraventricular nucleus (PVN), lateral hypothalamus (LH), and basolateral and central amygdala (BLA/CeA). For both ventral tegmental area (VTA) and medial preoptic area (MPOA) levels of Fabp7 were lower in mothers, but levels of changes did not reach significance. Confocal microscopy revealed that protein expression of Fabp7 in the LS paralleled mRNA findings. Specifically, the caudal LS exhibited a significant reduction in Fabp7 immunoreactivity, while decreases in medial LS were just above significance. Double fluorescent immunolabeling confirmed the astrocytic phenotype of Fabp7-expressing cells. Collectively, this research demonstrates a broad and marked reduction in Fabp7 expression in the postpartum brain, suggesting that down-regulation of Fabp7 may serve as a hallmark of the postpartum brain and contribute to the maternal phenotype.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chemical Neuroanatomy - Volume 92, October 2018, Pages 92-101
Journal: Journal of Chemical Neuroanatomy - Volume 92, October 2018, Pages 92-101
نویسندگان
Terri M. Driessen, Changjiu Zhao, Marissa Saenz, Sharon A. Stevenson, Yuji Owada, Stephen C. Gammie,